Description：Rabbit polyclonal antibody to P2X5Immunogen：Recombinant fusion protein of human P2X5. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：P2RX5Alternative Names：P2X5; P2X purinoceptor 5; P2X5; ATP receptor; Purinergic receptor Entrez Gene (Human)： 5026; Entrez Gene (Rat)： 113995; SwissProt (Human)： Q93086; SwissProt (Rat)： P51578; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of P2X5 expression in K562 (A), mouse kidney (B), rat heart (C) whole cell lysates. (Predicted band size: 44; 47; 48 kD; Observed band size: 115 kD)
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  Immunofluorescent analysis of P2X5 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).