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CMA4508
  • Western blot analysis of CD10 expression in H1792 (A), LO2 (B), mouse liver (C), mouse kidney (D), rat kidney (E) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 100 kD)
  • Immunohistochemical analysis of CD10 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
Product Name:Recombinant Anti-CD10 Rabbit mAb
Cat No:CMA4508
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
50 μl
150
30 μl
110
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Description:Recombinant rabbit monoclonal antibody to CD10
Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within human CD10 protein. The exact sequence is proprietary.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Monoclonal
Form:Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)
Gene Symbol:MME
Alternative Names:EPN; Neprilysin; Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; NEP; Neutral endopeptidase; Skin fibroblast elastase; SFE; CD10
Entrez Gene (Human): 4311;
Entrez Gene (Mouse): 17380;
Entrez Gene (Rat): 24590;
SwissProt (Human): P08473;
SwissProt (Mouse): Q61391;
SwissProt (Rat): P07861;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of CD10 expression in H1792 (A), LO2 (B), mouse liver (C), mouse kidney (D), rat kidney (E) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 100 kD)
  • Immunohistochemical analysis of CD10 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
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