Description：Rabbit polyclonal antibody to NSUN2Immunogen：Recombinant fusion protein of human NSUN2. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：NSUN2Alternative Names：SAKI; TRM4; tRNA (cytosine(34)-C(5))-methyltransferase; Myc-induced SUN domain-containing protein; Misu; NOL1/NOP2/Sun domain family member 2; Substrate of AIM1/Aurora kinase B; tRNA (cytosine-5-)-methyltransferase; tRNA methyltransferase 4 homolog; hTrm4 Entrez Gene (Human)： 54888; Entrez Gene (Mouse)： 28114; SwissProt (Human)： Q08J23; SwissProt (Mouse)： Q1HFZ0; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NSUN2 expression in A549 (A), mouse heart (B) whole cell lysates. (Predicted band size: 59; 82; 86 kD; Observed band size: 105 kD)
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  Immunofluorescent analysis of NSUN2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.