Description:Rabbit polyclonal antibody to Bex3Immunogen:Recombinant fusion protein of human Bex3. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:NGFRAP1Alternative Names:BEX3; DXS6984E; NADE; Protein BEX3; Brain-expressed X-linked protein 3; Nerve growth factor receptor-associated protein 1; Ovarian granulosa cell 13.0 kDa protein HGR74; p75NTR-associated cell death executor
Entrez Gene (Human):
27018;
Entrez Gene (Mouse):
12070;
Entrez Gene (Rat):
117089;
SwissProt (Human):
Q00994;
SwissProt (Mouse):
Q9WTZ9;
SwissProt (Rat):
Q6PDU5;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Bex3 expression in MCF7 (A), mouse liver (B) whole cell lysates. (Predicted band size: 11; 12 kD; Observed band size: 24 kD) -
Immunofluorescent analysis of Bex3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.


Datasheet
MSDS