Description:Rabbit polyclonal antibody to MRPS28Immunogen:Recombinant fusion protein of human MRPS28. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:MRPS28Alternative Names:MRPS35; 28S ribosomal protein S28 mitochondrial; MRP-S28; S28mt; 28S ribosomal protein S35 mitochondrial; MRP-S35; S35mt
Entrez Gene (Human):
28957;
Entrez Gene (Mouse):
66230;
SwissProt (Human):
Q9Y2Q9;
SwissProt (Mouse):
Q9CY16;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MRPS28 expression in HEK293T (A) whole cell lysates. (Predicted band size: 20 kD; Observed band size: 17 kD) -
Immunofluorescent analysis of MRPS28 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.


Datasheet
MSDS