Description：Rabbit polyclonal antibody to HSD17B8Immunogen：Recombinant fusion protein of human HSD17B8. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：HSD17B8Alternative Names：FABGL; HKE6; RING2; Estradiol 17-beta-dehydrogenase 8; 17-beta-hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; 3-oxoacyl-[acyl-carrier-protein] reductase; Protein Ke6; Ke-6; Really interesting new gene 2 protein; Testosterone 17-beta-dehydrogenase 8 Entrez Gene (Human)： 7923; Entrez Gene (Mouse)： 14979; Entrez Gene (Rat)： 361802; SwissProt (Human)： Q92506; SwissProt (Mouse)： P50171; SwissProt (Rat)： Q6MGB5; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HSD17B8 expression in HeLa (A), mouse liver (B), rat heart (C) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 27 kD)
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  Immunofluorescent analysis of HSD17B8 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.