Description：Rabbit polyclonal antibody to BF-1Immunogen：KLH-conjugated synthetic peptide of human BF-1. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：FOXG1Alternative Names：FKH2; FKHL1; FKHL2; FKHL3; FKHL4; FOXG1A; FOXG1B; FOXG1C; Forkhead box protein G1; Brain factor 1; BF-1; BF1; Brain factor 2; BF-2; BF2; hBF-2; Forkhead box protein G1A; Forkhead box protein G1B; Forkhead box protein G1C; Forkhead-related protein FKHL1; HFK1; Forkhead-related protein FKHL2; HFK2; Forkhead-related protein FKHL3; HFK3 Entrez Gene (Human)： 2290; Entrez Gene (Mouse)： 15228; Entrez Gene (Rat)： 24370; SwissProt (Human)： P55316; SwissProt (Mouse)： Q60987; SwissProt (Rat)： Q00939; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of BF-1 expression in HEK293T (A), mouse brain (B), mouse kidney (C), rat spleen (D) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 52 kD)
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  Immunofluorescent analysis of BF-1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.