Description：Rabbit polyclonal antibody to HIF PHD3Immunogen：Recombinant fusion protein of human HIF PHD3. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：EGLN3Alternative Names：Egl nine homolog 3; HPH-1; Hypoxia-inducible factor prolyl hydroxylase 3; HIF-PH3; HIF-prolyl hydroxylase 3; HPH-3; Prolyl hydroxylase domain-containing protein 3; PHD3 Entrez Gene (Human)： 112399; Entrez Gene (Mouse)： 112407; Entrez Gene (Rat)： 54702; SwissProt (Human)： Q9H6Z9; SwissProt (Mouse)： Q91UZ4; SwissProt (Rat)： Q62630; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HIF PHD3 expression in mouse liver (A) whole cell lysates. (Predicted band size: 27 kD; Observed band size: 32 kD)
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  Immunofluorescent analysis of HIF PHD3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.