Description：Rabbit polyclonal antibody to EEF1E1Immunogen：KLH-conjugated synthetic peptide of human EEF1E1. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：EEF1E1Alternative Names：AIMP3; P18; Eukaryotic translation elongation factor 1 epsilon-1; Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3; Elongation factor p18; Multisynthase complex auxiliary component p18 Entrez Gene (Human)： 9521; Entrez Gene (Mouse)： 66143; SwissProt (Human)： O43324; SwissProt (Mouse)： Q9D1M4; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EEF1E1 expression in HeLa (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 15; 19 kD; Observed band size: 20 kD)
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  Immunofluorescent analysis of EEF1E1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).