Description：Quick, efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications Directions for Use：Stock Solution:1L size: Dissolve the powder in 1L deionized water for Rapid Transfer Buffer (10X).100 ml size: Dissolve the powder in 100 ml deionized water for Rapid Transfer Buffer (10X).Working Solution:Prepare 1 L of Rapid Transfer Buffer (1X) by diluting 100 mL of Rapid Transfer Buffer (10X) with 800 mL of deionized water, then add 100 ml Ethanol.Application：Wet Transfer Protocol1. Prepare membrane and filter paper for transfer:a. A blotting membrane and 3 pieces of filter paper should be cut to fit dimensions of the gel.Note: PVDF membranes must be pre-wetted according to the manufacturer’s instructions in 100% methanol prior to equilibration in transfer buffer.b. Equilibrate the membrane and filter paper in 1X Rapid Transfer Buffer for a minimum of 5 minutes.2. Following protein electrophoresis, assemble the blotting sandwich following the manufacturer’s instructions for the transfer apparatus.3. Place the blotting sandwich in a wet transfer tank filled with 1X Rapid Transfer Buffer.4. Transfer constant current 300 - 400 mA at room temperature.Semi-Dry Transfer Protocol1. Prepare membrane and filter paper for transfer:a. A blotting membrane and 3 pieces of filter paper should be cut to fit dimensions of the gel.Note: PVDF membranes must be pre-wetted according to the manufacturer’s instructions in 100% methanol prior to equilibration in transfer buffer.b. Equilibrate the membrane and filter paper in 1X Rapid Transfer Buffer for a minimum of 5 minutes.2. Following electrophoresis, wash the gel for 2 minutes in deionized water.3. Pre-equilibrate the gel in 1X Rapid Transfer Buffer for 5 minutes.4. Assemble the blotting sandwich following the manufacturer’s instructions for the semi-dry transfer apparatus.5. Transfer constant current 300 - 400 mA at room temperature.Storage/Stability：Store at 25°C for 2 years.