Description:Rabbit polyclonal antibody to MRPL43Immunogen:Recombinant fusion protein of human MRPL43. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:MRPL43Alternative Names:39S ribosomal protein L43 mitochondrial; L43mt; MRP-L43; Mitochondrial ribosomal protein bMRP36a
Entrez Gene (Human):
84545;
SwissProt (Human):
Q8N983;
SwissProt (Mouse):
Q99N89;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MRPL43 expression in A549 (A), PC3 (B) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 17 kD) -
Immunofluorescent analysis of MRPL43 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).


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MSDS