Description:Rabbit polyclonal antibody to ARL8BImmunogen:Recombinant fusion protein of human ARL8B. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:ARL8BAlternative Names:ARL10C; GIE1; ADP-ribosylation factor-like protein 8B; ADP-ribosylation factor-like protein 10C; Novel small G protein indispensable for equal chromosome segregation 1
Entrez Gene (Human):
55207;
Entrez Gene (Mouse):
67166;
Entrez Gene (Rat):
500282;
SwissProt (Human):
Q9NVJ2;
SwissProt (Mouse):
Q9CQW2;
SwissProt (Rat):
Q66HA6;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ARL8B expression in U87MG (A), mouse brain (B) whole cell lysates. (Predicted band size: 18; 21 kD; Observed band size: 21 kD)
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Immunofluorescent analysis of ARL8B staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).