Description:Rabbit polyclonal antibody to ARL1Immunogen:Recombinant fusion protein of human ARL1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol:ARL1Alternative Names:ADP-ribosylation factor-like protein 1
Entrez Gene (Human):
400;
Entrez Gene (Mouse):
104303;
Entrez Gene (Rat):
64187;
SwissProt (Human):
P40616;
SwissProt (Mouse):
P61211;
SwissProt (Rat):
P61212;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ARL1 expression in A549 (A), mouse brain (B) whole cell lysates. (Predicted band size: 18; 20 kD; Observed band size: 20 kD)
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Immunofluorescent analysis of ARL1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.