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CQA3665
  • Western blot analysis of TRIP12 expression in Hela (A), Jurkat (B) whole cell lysates. (Predicted band size: 192; 220; 223; 225 kD; Observed band size: 250 kD)
  • Immunohistochemical analysis of TRIP12 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-TRIP12 Antibody
Cat No:CQA3665
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
50 μl
150
30 μl
110
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Description:Rabbit polyclonal antibody to TRIP12
Immunogen:Recombinant full length protein of human TRIP12
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)
Gene Symbol:TRIP12
Alternative Names:KIAA0045; ULF; E3 ubiquitin-protein ligase TRIP12; E3 ubiquitin-protein ligase for Arf; ULF; Thyroid receptor-interacting protein 12; TR-interacting protein 12; TRIP-12
Entrez Gene (Human): 9320;
Entrez Gene (Mouse): 14897;
Entrez Gene (Rat): 316575;
SwissProt (Human): Q14669;
SwissProt (Mouse): G5E870;
SwissProt (Rat): F1LP64;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of TRIP12 expression in Hela (A), Jurkat (B) whole cell lysates. (Predicted band size: 192; 220; 223; 225 kD; Observed band size: 250 kD)
  • Immunohistochemical analysis of TRIP12 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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