Description：Rabbit polyclonal antibody to HSD3B1Immunogen：Recombinant full length protein of human HSD3B1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：HSD3B1Alternative Names：3BH; HSDB3A; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; Trophoblast antigen FDO161G Entrez Gene (Human)： 3283; Entrez Gene (Mouse)： 15492; Entrez Gene (Rat)： 360348; SwissProt (Human)： P14060; SwissProt (Mouse)： P24815; SwissProt (Rat)： P22071; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HSD3B1 expression in A375 (A) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)
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  Immunofluorescent analysis of HSD3B1 staining in human placenta. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).