Description:Rabbit polyclonal antibody to IKK epsilonImmunogen:Recombinant full length protein of human IKK epsilonPurification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)Gene Symbol:IKBKEAlternative Names:IKKE; IKKI; KIAA0151; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; Inducible I kappa-B kinase; IKK-i
Entrez Gene (Human):
9641;
Entrez Gene (Mouse):
56489;
SwissProt (Human):
Q14164;
SwissProt (Mouse):
Q9R0T8;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of IKK epsilon expression in SW480 (A), MCF7 (B), mouse liver (C) whole cell lysates. (Predicted band size: 70; 80 kD; Observed band size: 93 kD)
-
Immunohistochemical analysis of IKK epsilon staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.