Description：Rabbit polyclonal antibody to DNAJA3Immunogen：Recombinant full length protein of human DNAJA3Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)Gene Symbol：DNAJA3Alternative Names：HCA57; TID1; DnaJ homolog subfamily A member 3 mitochondrial; DnaJ protein Tid-1; hTid-1; Hepatocellular carcinoma-associated antigen 57; Tumorous imaginal discs protein Tid56 homolog Entrez Gene (Human)： 9093; Entrez Gene (Mouse)： 83945; SwissProt (Human)： Q96EY1; SwissProt (Mouse)： Q99M87; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DNAJA3 expression in Jurkat (A), HepG2 (B), mouse kidney (C), mouse heart (D), rat liver (E) whole cell lysates. (Predicted band size: 33; 49; 52 kD; Observed band size: 52 kD)
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  Immunofluorescent analysis of DNAJA3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.