Description:Rabbit polyclonal antibody to Transgelin-2Immunogen:Recombinant full length protein of human Transgelin-2Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:TAGLN2Alternative Names:KIAA0120; Transgelin-2; Epididymis tissue protein Li 7e; SM22-alpha homolog
Entrez Gene (Human):
8407;
Entrez Gene (Mouse):
21346;
Entrez Gene (Rat):
304983;
SwissProt (Human):
P37802;
SwissProt (Mouse):
Q9WVA4;
SwissProt (Rat):
Q5XFX0;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Transgelin-2 expression in HEK293T (A), Hela (B), THP1 (C), MCF7 (D), SKOV3 (E), mouse lung (F) whole cell lysates. (Predicted band size: 22; 24 kD; Observed band size: 22 kD)
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Immunofluorescent analysis of Transgelin-2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.