Description:Rabbit polyclonal antibody to DARSImmunogen:Recombinant full length protein of human DARSPurification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol:DARSAlternative Names:Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein
Entrez Gene (Human):
1615;
Entrez Gene (Mouse):
226414;
Entrez Gene (Rat):
116483;
SwissProt (Human):
P14868;
SwissProt (Mouse):
Q922B2;
SwissProt (Rat):
P15178;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DARS expression in MCF7 (A), SW480 (B), mouse brain (C), rat liver (D) whole cell lysates. (Predicted band size: 45; 57 kD; Observed band size: 57 kD)
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Immunohistochemical analysis of DARS staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.