Description:Rabbit polyclonal antibody to DDAH2Immunogen:Recombinant full length protein of human DDAH2Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol:DDAH2Alternative Names:DDAH; G6A; NG30; N(G),N(G)-dimethylarginine dimethylaminohydrolase 2; DDAH-2; Dimethylarginine dimethylaminohydrolase 2; DDAHII; Dimethylargininase-2; Protein G6a; S-phase protein
Entrez Gene (Human):
23564;
Entrez Gene (Mouse):
51793;
Entrez Gene (Rat):
294239;
SwissProt (Human):
O95865;
SwissProt (Mouse):
Q99LD8;
SwissProt (Rat):
Q6MG60;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DDAH2 expression in SW620 (A), MCF7 (B), mouse lung (C), mouse kidney (D), rat heart (E), rat brain (F) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 30 kD)
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Immunofluorescent analysis of DDAH2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.