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CQA1363
  • Western blot analysis of PPOX expression in U2OS (A) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 51 kD)
  • Immunohistochemical analysis of PPOX staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-PPOX Antibody
Cat No:CQA1363
Source:Rabbit
Reactivity:H, M
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
50 μl
150
30 μl
110
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Description:Rabbit polyclonal antibody to PPOX
Immunogen:Recombinant full length protein of human PPOX
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200)
Gene Symbol:PPOX
Alternative Names:Protoporphyrinogen oxidase; PPO
Entrez Gene (Human): 5498;
Entrez Gene (Mouse): 19044;
SwissProt (Human): P50336;
SwissProt (Mouse): P51175;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of PPOX expression in U2OS (A) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 51 kD)
  • Immunohistochemical analysis of PPOX staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Heterologous expression and purification of recombinant human protoporphyrinogen oxidase IX: A comparative study
Journal PLOS ONE
IF 3.24
Application WB
Reactivity Human
PMID 34793488
Generation and characterization of human U-2 OS cell lines with the CRISPR/Cas9-edited protoporphyrinogen oxidase IX gene
Journal Research Square
IF
Application WB
Reactivity Human
PMID 36224252
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