Description：Rabbit polyclonal antibody to Pinch-1Immunogen：Recombinant full length protein of human Pinch-1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)Gene Symbol：LIMS1Alternative Names：PINCH; PINCH1; LIM and senescent cell antigen-like-containing domain protein 1; Particularly interesting new Cys-His protein 1; PINCH-1; Renal carcinoma antigen NY-REN-48 Entrez Gene (Human)： 3987; Entrez Gene (Mouse)： 110829; SwissProt (Human)： P48059; SwissProt (Mouse)： Q99JW4; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Pinch-1 expression in HepG2 (A) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 40 kD)
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  Immunofluorescent analysis of Pinch-1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.