Description:Rabbit polyclonal antibody to Neurokinin BImmunogen:Recombinant full length protein of human Neurokinin BPurification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)Gene Symbol:TAC3Alternative Names:NKNB; Tachykinin-3; ZNEUROK1
Entrez Gene (Human):
6866;
Entrez Gene (Mouse):
21334;
Entrez Gene (Rat):
29191;
SwissProt (Human):
Q9UHF0;
SwissProt (Mouse):
P55099;
SwissProt (Rat):
P08435;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Neurokinin B expression in Jurkat (A), mouse spleen (B), rat kindney (C), rat brain (D) whole cell lysates. (Predicted band size: 11; 13; 14 kD; Observed band size: 16 kD)
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Immunofluorescent analysis of Neurokinin B staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.