Description：Rabbit polyclonal antibody to PDC-E2Immunogen：Recombinant full length protein of human PDC-E2Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/10 - 1/100)Gene Symbol：DLATAlternative Names：DLTA; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; PBC; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; Pyruvate dehydrogenase complex component E2; PDC-E2; PDCE2 Entrez Gene (Human)： 1737; Entrez Gene (Mouse)： 235339; Entrez Gene (Rat)： 81654; SwissProt (Human)： P10515; SwissProt (Mouse)： Q8BMF4; SwissProt (Rat)： P08461; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PDC-E2 expression in HepG2 (A), Jurkat (B), mouse heart (C), rat brain (D), rat kidney (E) whole cell lysates. (Predicted band size: 68 kD; Observed band size: 69 kD)
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  Immunofluorescent analysis of PDC-E2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.