Description：Rabbit polyclonal antibody to Fis1Immunogen：Recombinant full length protein of human Fis1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/50)Gene Symbol：FIS1Alternative Names：TTC11; Mitochondrial fission 1 protein; FIS1 homolog; hFis1; Tetratricopeptide repeat protein 11; TPR repeat protein 11 Entrez Gene (Human)： 51024; Entrez Gene (Mouse)： 66437; Entrez Gene (Rat)： 288584; SwissProt (Human)： Q9Y3D6; SwissProt (Mouse)： Q9CQ92; SwissProt (Rat)： P84817; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Fis1 expression in HepG2 (A), A549 (B), mouse spleen (C), mouse kidney (D) whole cell lysates. (Predicted band size: 16 kD; Observed band size: 16 kD)
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  Immunofluorescent analysis of Fis1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.