Description:Rabbit polyclonal antibody to CGBPImmunogen:Recombinant full length protein of human CGBPPurification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:CXXC1Alternative Names:CFP1; CGBP; PCCX1; PHF18; CXXC-type zinc finger protein 1; CpG-binding protein; PHD finger and CXXC domain-containing protein 1
Entrez Gene (Human):
30827;
Entrez Gene (Mouse):
74322;
SwissProt (Human):
Q9P0U4;
SwissProt (Mouse):
Q9CWW7;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of CGBP expression in HepG2 (A), Hela (B), A431 (C), mouse spleen (D), rat testis (E) whole cell lysates. (Predicted band size: 75; 76 kD; Observed band size: 75 kD)
-
Immunofluorescent analysis of CGBP staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.