Description：Rabbit polyclonal antibody to Thrombospondin-5Immunogen：Recombinant full length protein of human Thrombospondin-5Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：COMPAlternative Names：Cartilage oligomeric matrix protein; COMP; Thrombospondin-5; TSP5 Entrez Gene (Human)： 1311; Entrez Gene (Mouse)： 12845; Entrez Gene (Rat)： 25304; SwissProt (Human)： P49747; SwissProt (Mouse)： Q9R0G6; SwissProt (Rat)： P35444; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Thrombospondin-5 expression in HepG2 (A), MCF7 (B), mouse heart (C), mouse ovary (D) whole cell lysates. (Predicted band size: 77; 82 kD; Observed band size: 110 kD)
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  Immunofluorescent analysis of Thrombospondin-5 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).