Datasheet
MSDSDescription：Rabbit polyclonal antibody to ATP5BImmunogen：Recombinant full length protein of human ATP5BPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：ATP5BAlternative Names：ATPMB; ATPSB; ATP synthase subunit beta mitochondrial
Entrez Gene (Human)：
506;
Entrez Gene (Mouse)：
11947;
Entrez Gene (Rat)：
171374;
SwissProt (Human)：
P06576;
SwissProt (Mouse)：
P56480;
SwissProt (Rat)：
P10719;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ATP5B expression in SW620 (A), HepG2 (B), mouse kidney (C), mouse brain (D) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 52 kD) -
Immunofluorescent analysis of ATP5B staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).