Description：Rabbit polyclonal antibody to PA28 alphaImmunogen：Recombinant full length protein of human PA28 alphaPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：PSME1Alternative Names：IFI5111; Proteasome activator complex subunit 1; 11S regulator complex subunit alpha; REG-alpha; Activator of multicatalytic protease subunit 1; Interferon gamma up-regulated I-5111 protein; IGUP I-5111; Proteasome activator 28 subunit alpha; PA28a; PA28alpha Entrez Gene (Human)： 5720; Entrez Gene (Mouse)： 19186; SwissProt (Human)： Q06323; SwissProt (Mouse)： P97371; SwissProt (Rat)： Q63797; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PA28 alpha expression in MCF7 (A), BT474 (B), mouse spleen (C), mouse liver (D) whole cell lysates. (Predicted band size: 26; 28 kD; Observed band size: 28 kD)
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  Immunofluorescent analysis of PA28 alpha staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.