Description：Rabbit polyclonal antibody to GM130Immunogen：Recombinant full length protein of human GM130Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：GOLGA2Alternative Names：Golgin subfamily A member 2; 130 kDa cis-Golgi matrix protein; GM130; GM130 autoantigen; Golgin-95 Entrez Gene (Human)： 2801; Entrez Gene (Mouse)： 99412; Entrez Gene (Rat)： 64528; SwissProt (Human)： Q08379; SwissProt (Mouse)： Q921M4; SwissProt (Rat)： Q62839; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GM130 expression in Raji (A), Hela (B) whole cell lysates. (Predicted band size: 70; 113 kD; Observed band size: 130 kD)
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  Immunofluorescent analysis of GM130 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.