Description：Rabbit polyclonal antibody to IRSp53Immunogen：Recombinant full length protein of human IRSp53Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：BAIAP2Alternative Names：Brain-specific angiogenesis inhibitor 1-associated protein 2; BAI-associated protein 2; BAI1-associated protein 2; Protein BAP2; Fas ligand-associated factor 3; FLAF3; Insulin receptor substrate p53/p58; IRS-58; IRSp53/58; Insulin receptor substrate protein of 53 kDa; IRSp53; Insulin receptor substrate p53 Entrez Gene (Human)： 10458; Entrez Gene (Mouse)： 108100; Entrez Gene (Rat)： 117542; SwissProt (Human)： Q9UQB8; SwissProt (Mouse)： Q8BKX1; SwissProt (Rat)： Q6GMN2; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of IRSp53 expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 56; 57; 59; 60 kD; Observed band size: 53; 58 kD)
• 
  Immunofluorescent analysis of IRSp53 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.