Description:Rabbit polyclonal antibody to PSMC4Immunogen:Recombinant full length protein of human PSMC4Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:PSMC4Alternative Names:MIP224; TBP7; 26S protease regulatory subunit 6B; 26S proteasome AAA-ATPase subunit RPT3; MB67-interacting protein; MIP224; Proteasome 26S subunit ATPase 4; Tat-binding protein 7; TBP-7
Entrez Gene (Human):
5704;
Entrez Gene (Mouse):
23996;
Entrez Gene (Rat):
117262;
SwissProt (Human):
P43686;
SwissProt (Mouse):
P54775;
SwissProt (Rat):
Q63570;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PSMC4 expression in HepG2 (A), A549 (B), Hela (C), Jurkat (D), mouse liver (E) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)
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Immunofluorescent analysis of PSMC4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.