Description:Rabbit polyclonal antibody to PSMA2Immunogen:Recombinant full length protein of human PSMA2Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:PSMA2Alternative Names:HC3; PSC3; Proteasome subunit alpha type-2; Macropain subunit C3; Multicatalytic endopeptidase complex subunit C3; Proteasome component C3
Entrez Gene (Human):
5683;
Entrez Gene (Mouse):
19166;
Entrez Gene (Rat):
29669;
SwissProt (Human):
P25787;
SwissProt (Mouse):
P49722;
SwissProt (Rat):
P17220;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PSMA2 expression in Jurkat (A), Hela (B), NIH3T3 (C), mouse brain (D) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 26 kD)
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Immunofluorescent analysis of PSMA2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.