Description:Rabbit polyclonal antibody to EDF1Immunogen:Recombinant full length protein of human EDF1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol:EDF1Alternative Names:Endothelial differentiation-related factor 1; EDF-1; Multiprotein-bridging factor 1; MBF1
Entrez Gene (Human):
8721;
Entrez Gene (Mouse):
59022;
Entrez Gene (Rat):
296570;
SwissProt (Human):
O60869;
SwissProt (Mouse):
Q9JMG1;
SwissProt (Rat):
P69736;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EDF1 expression in HepG2 (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 15; 16 kD; Observed band size: 16 kD)
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Immunohistochemical analysis of EDF1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.