Description：Rabbit polyclonal antibody to PSMB5Immunogen：Recombinant full length protein of human PSMB5Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/50)Gene Symbol：PSMB5Alternative Names：LMPX; MB1; X; Proteasome subunit beta type-5; Macropain epsilon chain; Multicatalytic endopeptidase complex epsilon chain; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit MB1; Proteasome subunit X Entrez Gene (Human)： 5693; Entrez Gene (Mouse)： 19173; Entrez Gene (Rat)： 29425; SwissProt (Human)： P28074; SwissProt (Mouse)： O55234; SwissProt (Rat)： P28075; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PSMB5 expression in Jurkat (A), HepG2 (B), mouse brain (C), mouse kidney (D) whole cell lysates. (Predicted band size: 17; 21; 28 kD; Observed band size: 21 kD)
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  Immunofluorescent analysis of PSMB5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.