Description：Rabbit polyclonal antibody to U2AF65Immunogen：Recombinant full length protein of human U2AF65Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：U2AF2Alternative Names：U2AF65; Splicing factor U2AF 65 kDa subunit; U2 auxiliary factor 65 kDa subunit; hU2AF(65); hU2AF65; U2 snRNP auxiliary factor large subunit Entrez Gene (Human)： 11338; Entrez Gene (Mouse)： 22185; SwissProt (Human)： P26368; SwissProt (Mouse)： P26369; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of U2AF65 expression in Jurkat (A), SW620 (B), mouse spleen (C) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 54 kD)
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  Immunofluorescent analysis of U2AF65 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.