Description:Rabbit polyclonal antibody to CBSImmunogen:Recombinant full length protein of human CBSPurification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:CBSAlternative Names:Cystathionine beta-synthase; Beta-thionase; Serine sulfhydrase
Entrez Gene (Human):
102724560;
875;
Entrez Gene (Mouse):
12411;
Entrez Gene (Rat):
24250;
SwissProt (Human):
P35520;
SwissProt (Mouse):
Q91WT9;
SwissProt (Rat):
P32232;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CBS expression in Hela (A), MCF7 (B), Romas (C), mouse brain (D) whole cell lysates. (Predicted band size: 60; 61 kD; Observed band size: 61 kD)
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Immunofluorescent analysis of CBS staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.