Description：Rabbit polyclonal antibody to FibrillarinImmunogen：Recombinant full length protein of human FibrillarinPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200), IP (1/20 - 1/50)Gene Symbol：FBLAlternative Names：FIB1; FLRN; rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; Histone-glutamine methyltransferase Entrez Gene (Human)： 2091; Entrez Gene (Mouse)： 102643269; 14113; Entrez Gene (Rat)： 292747; SwissProt (Human)： P22087; SwissProt (Mouse)： P35550; SwissProt (Rat)： P22509; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Fibrillarin expression in Hela (A), U20S (B), COS7 (C), PC12 (D) whole cell lysates. (Predicted band size: 33 kD; Observed band size: 37 kD)
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  Immunofluorescent analysis of Fibrillarin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.