Description:Rabbit polyclonal antibody to RhoCImmunogen:Recombinant full length protein of human RhoCPurification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol:RHOCAlternative Names:ARH9; ARHC; Rho-related GTP-binding protein RhoC; Rho cDNA clone 9; h9
Entrez Gene (Human):
389;
Entrez Gene (Mouse):
11853;
SwissProt (Human):
P08134;
SwissProt (Mouse):
Q62159;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of RhoC expression in Jurkat (A), A549 (B), PC12 (C) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 22 kD)
-
Immunofluorescent analysis of RhoC staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.