Description:Rabbit polyclonal antibody to EEF1A1Immunogen:Recombinant full length protein of human EEF1A1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/20 - 1/100)Gene Symbol:EEF1A1Alternative Names:EEF1A; EF1A; LENG7; Elongation factor 1-alpha 1; EF-1-alpha-1; Elongation factor Tu; EF-Tu; Eukaryotic elongation factor 1 A-1; eEF1A-1; Leukocyte receptor cluster member 7
Entrez Gene (Human):
1915;
Entrez Gene (Mouse):
13627;
Entrez Gene (Rat):
171361;
SwissProt (Human):
P68104;
SwissProt (Mouse):
P10126;
SwissProt (Rat):
P62630;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EEF1A1 expression in MCF7 (A), Jurkat (B), HepG2 (C), SHSY5Y (D) whole cell lysates. (Predicted band size: 47; 50 kD; Observed band size: 50 kD)
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Immunofluorescent analysis of EEF1A1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.