Description:Rabbit polyclonal antibody to Calpain 1Immunogen:Recombinant full length protein of human Calpain 1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol:CAPN1Alternative Names:CANPL1; Calpain-1 catalytic subunit; Calcium-activated neutral proteinase 1; CANP 1; Calpain mu-type; Calpain-1 large subunit; Cell proliferation-inducing gene 30 protein; Micromolar-calpain; muCANP
Entrez Gene (Human):
823;
Entrez Gene (Mouse):
12333;
Entrez Gene (Rat):
29153;
SwissProt (Human):
P07384;
SwissProt (Mouse):
O35350;
SwissProt (Rat):
P97571;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Calpain 1 expression in A549 (A), MCF7 (B), Jurkat (C) whole cell lysates. (Predicted band size: 81 kD; Observed band size: 75-80 kD)
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Immunohistochemical analysis of Calpain 1 staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.