Description:Mouse monoclonal antibody to Actin Sarcomeric MuscleImmunogen:KLH-conjugated synthetic peptide encompassing a sequence within human Actin Sarcomeric Muscle. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:MonoclonalForm:Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution:IH (1/100 - 1/300)Gene Symbol:ACTA1; ACTC1Alternative Names:ACTA; Actin alpha skeletal muscle; Alpha-actin-1; ACTC; Actin alpha cardiac muscle 1; Alpha-cardiac actin
Entrez Gene (Human):
58;
70;
SwissProt (Human):
P68133;
P68032;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Immunohistochemical analysis of Actin Sarcomeric Muscle staining in human myocardium formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunohistochemical analysis of Actin Sarcomeric Muscle staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.