Description:Mouse monoclonal antibody to Collagen 3 alpha 1Immunogen:KLH-conjugated synthetic peptide encompassing a sequence within human Collagen 3 alpha 1. The exact sequence is proprietary.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:MonoclonalForm:Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IH (1/100 - 1/300), IF (1/100 - 1/500)Gene Symbol:COL3A1Alternative Names:Collagen alpha-1(III) chain
Entrez Gene (Human):
1281;
SwissProt (Human):
P02461;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Collagen 3 alpha 1 expression in Hela (A) whole cell lysates. (Predicted band size: 138 kD; Observed band size: 180 kD)
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Immunohistochemical analysis of Collagen 3 alpha 1 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.