Description：Mouse monoclonal antibody to MLH1Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within human MLH1. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：MonoclonalForm：Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/300), IF (1/100 - 1/500)Gene Symbol：MLH1Alternative Names：COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1 Entrez Gene (Human)： 4292; SwissProt (Human)： P40692; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MLH1 expression in K562 (A), HEK293 (B), Hela (C) whole cell lysates. (Predicted band size: 84 kD; Observed band size: 88 kD)
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  Immunohistochemical analysis of MLH1 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunohistochemical analysis of MLH1 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.