Description:Rabbit polyclonal antibody to TGF beta 1Immunogen:Recombinant protein corresponding to human TGF beta 1.Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/1000 - 1/2000), IH (1/200 - 1/500)Gene Symbol:TGFB1Alternative Names:TGFB; Transforming growth factor beta-1; TGF-beta-1
Entrez Gene (Human):
7040;
SwissProt (Human):
P01137;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TGF beta 1 expression in mouse liver (A), rat liver (B) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 12; 25; 45-65 kD)
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Immunohistochemical analysis of TGF beta 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.