Description：Rabbit polyclonal antibody to Histone H3 (Citruline-R2+R8+R17)Immunogen：KLH-conjugated synthetic citrullinated peptide corresponding to residues surrounding R2+R8+R17 of human Histone H3 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3JAlternative Names：H3FA; H3FL; H3FC; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l Entrez Gene (Human)： 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968; Entrez Gene (Mouse)： 319152; 15077; 15078; Entrez Gene (Rat)： 291159; 100361558; SwissProt (Human)： P68431; Q71DI3; P84243; SwissProt (Mouse)： P68433; P84228; P84244; SwissProt (Rat)： Q6LED0; P84245; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H3 (Citruline-R2+R8+R17) expression in Hela (A), NIH3T3 (B) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)
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  Immunohistochemical analysis of Histone H3 (Citruline-R2+R8+R17) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Histone H3 (Citruline-R2+R8+R17) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).