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CPA9247
  • Western blot analysis of Beta-tubulin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 55 kD)
  • Immunohistochemical analysis of Beta-tubulin staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-Beta-tubulin Antibody
Cat No:CPA9247
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
130
100 μl
80
30 μl
40
50 μl
55
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Description:Rabbit polyclonal antibody to Beta-tubulin
Immunogen:Recombinant protein corresponding to human Beta-tubulin.
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 0.2% BSA, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/3000 - 1/10000), IH (1/50 - 1/100)
Gene Symbol:TUBB3
Alternative Names:TUBB4; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III
Entrez Gene (Human): 10381;
Entrez Gene (Mouse): 22152;
Entrez Gene (Rat): 246118;
SwissProt (Human): Q13509;
SwissProt (Mouse): Q9ERD7;
SwissProt (Rat): Q4QRB4;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Beta-tubulin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 55 kD)
  • Immunohistochemical analysis of Beta-tubulin staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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