Datasheet
MSDSDescription：Mouse monoclonal antibody to TNF alphaImmunogen：Recombinant protein corresponding to human TNF alpha.Purification：Affinity chromatographyClonality：MonoclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/1000 - 1/2000), IH (1/50 - 1/200)Gene Symbol：TNFAlternative Names：TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Entrez Gene (Human)：
7124;
Entrez Gene (Mouse)：
21926;
Entrez Gene (Rat)：
24835;
SwissProt (Human)：
P01375;
SwissProt (Mouse)：
P06804;
SwissProt (Rat)：
P16599;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TNF alpha expression in recombinant human TNF-a protein (A) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 17; 25 kD) -
Immunohistochemical analysis of TNF alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.