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CPA9214
  • Western blot analysis of STAT3 expression in Hela (A), mouse heart (B), rat heart (C) whole cell lysates. (Predicted band size: 88 kD; Observed band size: 79; 86 kD)
  • Immunohistochemical analysis of STAT3 staining in human colon cancer,mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-STAT3 Antibody
Cat No:CPA9214
Source:Mouse
Reactivity:H, M, R
Applications:WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
30 μl
110
50 μl
150
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Description:Mouse monoclonal antibody to STAT3
Immunogen:Recombinant protein corresponding to human STAT3.
Purification:Affinity chromatography
Clonality:Monoclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Gene Symbol:STAT3
Alternative Names:APRF; Signal transducer and activator of transcription 3; Acute-phase response factor
Entrez Gene (Human): 6774;
SwissProt (Human): P40763;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of STAT3 expression in Hela (A), mouse heart (B), rat heart (C) whole cell lysates. (Predicted band size: 88 kD; Observed band size: 79; 86 kD)
  • Immunohistochemical analysis of STAT3 staining in human colon cancer,mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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