Description：Mouse monoclonal antibody to Smooth Muscle ActinImmunogen：KLH-conjugated synthetic peptide encompassing a sequence of human Smooth Muscle Actin. The exact sequence is proprietary.Purification：Affinity chromatographyClonality：MonoclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/10000 - 1/100000), IH (1/100 - 1/1000)Gene Symbol：ACTA2Alternative Names：ACTSA; ACTVS; Actin aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein Entrez Gene (Human)： 59; SwissProt (Human)： P62736; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Smooth Muscle Actin expression in Hela (A), NIH3T3 (B), rat brain (C) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)
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  Immunohistochemical analysis of Smooth Muscle Actin staining in mouse cecal formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunohistochemical analysis of alpha Smooth Muscle Actin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.